Published May 4, 2019 | Version v1
Publication Open

Anti-Influenza a virus of a new oligosaccharide citric acid derivative isolated from<i>Vigna angularis</i>(ohwi<i>et</i>ohashi. var. Dainagon) Seeds

Creators

  • 1. National Research Centre
  • 2. Nagasaki University

Description

A new oligosaccharide citric acid derivative was isolated by hot aqueous extraction at 85 °C from Vigna angularis seeds, and it was purified using anion exchange chromatography DE-52. The structure was inferred by using UV, 1 D and 2 D NMR spectroscopy and HR-MALDI/MS, and identified as α-D-galactopyranosyl-(1→6)-α-D-galactopyranosyl-(1→6)-α-D-glucopyranosyle-(1→2)-β-D-fructofuranosyle citric acid. The isolated oligosaccharide citric acid derivative revealed promising anti-influenza A virus with 50% inhibition concentration (IC50) of 0.162 nM/mL and 50% cytotoxic dose (CC50) of >2.38 nM/mL.

⚠️ This is an automatic machine translation with an accuracy of 90-95%

Translated Description (Arabic)

تم عزل مشتق حمض الستريك أوليجو سكاريد جديد عن طريق الاستخراج المائي الساخن عند 85 درجة مئوية من بذور Vigna الزاوي، وتم تنقيته باستخدام كروماتوغرافيا تبادل الأنيون DE -52. تم الاستدلال على الهيكل باستخدام التحليل الطيفي بالأشعة فوق البنفسجية، 1 D و 2 D NMR و HR - MALDI/MS، وتم تحديده على أنه α - D - galactopyranosyl - (1 6) - α - D - galactopyranosyl - (1→→6) - α - D - glucopyranosyle - (1→2) - β - D - fructofuranosyle حامض الستريك. كشف مشتق حمض الستريك قليل السكاريد المعزول عن فيروس واعد مضاد للإنفلونزا أ بتركيز تثبيط 50 ٪ (IC50) قدره 0.162 نانومتر/مل و 50 ٪ جرعة سامة للخلايا (CC50) >2.38 نانومتر/مل.

Translated Description (French)

Un nouveau dérivé d'acide citrique oligosaccharidique a été isolé par extraction aqueuse à chaud à 85 °C à partir de graines de Vigna angularis, et il a été purifié en utilisant la chromatographie d'échange d'anions DE-52. La structure a été déduite en utilisant la spectroscopie RMN UV, 1 D et 2 D et HR-MALDI/MS, et identifiée comme étant l'→acide citrique α-D-galactopyranosyl- (1→6) -α-D-galactopyranosyl- (1 6) -α-D-glucopyranosyle- (1→2) -β-D-fructofuranosyle. Le dérivé d'acide citrique oligosaccharidique isolé a révélé un virus antigrippal A prometteur avec une concentration d'inhibition de 50 % (CI50) de 0,162 nM/mL et une dose cytotoxique de 50 % (CC50) >2,38 nM/mL.

Translated Description (Spanish)

Se aisló un nuevo derivado de ácido cítrico de oligosacárido mediante extracción acuosa en caliente a 85 °C de semillas de Vigna angularis, y se purificó usando cromatografía de intercambio aniónico DE-52. La estructura se dedujo mediante el uso de espectroscopía de RMN UV, 1 D y 2 D y HR-MALDI/MS, y se identificó como ácido cítrico→ α-D-galactopiranosil- (1→6) -α-D-galactopiranosil- (1 6) -α-D-glucopiranosilo- (1→2) -β-D-fructofuranosilo. El derivado de ácido cítrico de oligosacárido aislado reveló un virus antigripal A prometedor con una concentración de inhibición del 50% (IC50) de 0,162 nM/mL y una dosis citotóxica del 50% (CC50) de >2,38 nM/mL.

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Additional details

Additional titles

Translated title (Arabic)
مضاد للإنفلونزا فيروس من مشتق حمض الستريك قليل السكاريد الجديد المعزول من بذور<i> Vigna</i>angularis (<i>ohwi et</i> ohashi. var. Dainagon)
Translated title (French)
Anti-Influenza a virus d'un nouveau dérivé d'acide citrique oligosaccharidique isolé à partir de graines de<i>Vigna angularis</i>(ohwi<i>et</i> ohashi. var. Dainagon)
Translated title (Spanish)
Virus antiinfluenza de un nuevo oligosacárido derivado del ácido cítrico aislado de semillas de<i>Vigna angularis</i>(<i>ohwi et</i> ohashi. var. Dainagon)

Identifiers

Other
https://openalex.org/W2948022651
DOI
10.1080/07328303.2019.1615499

Is supplement to
https://openalex.org/W4206004635 (Other)
https://openalex.org/W322528242 (Other)
https://openalex.org/W3088327021 (Other)
https://openalex.org/W2980666398 (Other)
https://openalex.org/W2774981922 (Other)
https://openalex.org/W2497919145 (Other)
https://openalex.org/W2321165654 (Other)
https://openalex.org/W2310914630 (Other)
https://openalex.org/W2177714695 (Other)
https://openalex.org/W1471825263 (Other)

GreSIS Basics Section

Is Global South Knowledge
Yes
Country
Egypt

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