Published March 1, 2022
| Version v1
Publication
A new method for preparing single-cell nuclear suspension of frozen spinal cord tissue
Creators
- 1. The First Affiliated Hospital, Sun Yat-sen University
- 2. Sun Yat-sen University
- 3. The Seventh Affiliated Hospital of Sun Yat-sen University
- 4. AO Foundation
- 5. Beijing Jishuitan Hospital
- 6. Peking University
- 7. Shenzhen Second People's Hospital
- 8. Shenzhen University Health Science Center
Description
• A new method for preparing high-quality single-cell nuclear suspension of frozen spinal cord issue. • Tissue beating method breaks the colloidal scar and facilitates full grinding. • Two density gradient centrifugation were performed to remove impurities.
Translated Descriptions
⚠️
This is an automatic machine translation with an accuracy of 90-95%
Translated Description (Arabic)
• طريقة جديدة لتحضير معلق نووي أحادي الخلية عالي الجودة لمشكلة النخاع الشوكي المتجمد. • طريقة ضرب الأنسجة تكسر الندبة الغروانية وتسهل الطحن الكامل. • تم إجراء طرد مركزي متدرج الكثافة لإزالة الشوائب.Translated Description (French)
• Une nouvelle méthode pour préparer une suspension nucléaire monocellulaire de haute qualité d'un problème de moelle épinière congelée. • La méthode de battage des tissus casse la cicatrice colloïdale et facilite le meulage complet. • Une centrifugation à deux gradients de densité a été effectuée pour éliminer les impuretés.Translated Description (Spanish)
• Un nuevo método para preparar una suspensión nuclear unicelular de alta calidad de un problema de médula espinal congelada. • El método de batido de tejidos rompe la cicatriz coloidal y facilita la molienda completa. • Se realizaron dos centrifugaciones en gradiente de densidad para eliminar las impurezas.Additional details
Additional titles
- Translated title (Arabic)
- طريقة جديدة لتحضير المعلق النووي أحادي الخلية لأنسجة الحبل الشوكي المجمدة
- Translated title (French)
- Une nouvelle méthode de préparation d'une suspension nucléaire unicellulaire de tissu médullaire congelé
- Translated title (Spanish)
- Un nuevo método para preparar una suspensión nuclear unicelular de tejido congelado de la médula espinal
Identifiers
- Other
- https://openalex.org/W4206964814
- DOI
- 10.1016/j.jneumeth.2022.109490
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