Published April 1, 2004
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Influência de Tween na produção de lacases constitutivas e indutivas pelo Botryosphaeria sp
- 1. Unidad Ejecutora Lillo
Description
Botryosphaeria sp. is a ligninolytic fungus that produces laccase. It was previously established that veratryl alcohol (VA) strongly induced laccase production in this ascomyceteous. It is well known that surfactants such as Tween - 80 can increase the production of some fungal exo-enzymes. It has been reported that Tween-80 can also induce laccase production in basidiomycetes. In this work, the influence of Tweens 20, 40, 60 and 80 on the production of constitutive laccases (in the absence of VA) and inducible laccases (in the presence of VA) by Botryosphaeria sp. was evaluated, separately. Laccase activity was assayed using two putative laccase substrates: ABTS (PPO - I) and DMP (PPO - II). All of the Tweens examined increased the production of both constitutive and inducible laccase PPO - I, but did not affect the production of constitutive laccase PPO - II. Tweens 60 and 20 increased the production of inducible laccase PPO - II, while Tween 80 and 40 did not cause any effect
Translated Descriptions
⚠️
This is an automatic machine translation with an accuracy of 90-95%
Translated Description (Arabic)
Botryosphaeria sp. is a ligninolytic fungus that produce laccase. وقد ثبت سابقًا أن كحول الفيراتريل (VA) يحفز بقوة إنتاج اللاكيز في هذا النوع من الفطريات. من المعروف أن المواد الخافضة للتوتر السطحي مثل توين - 80 يمكن أن تزيد من إنتاج بعض الإنزيمات الخارجية الفطرية. تم الإبلاغ عن أن Tween -80 يمكن أن يحفز أيضًا إنتاج اللاكيز في الفطريات القاعدية. في هذا العمل، تم تقييم تأثير Tweens 20 و 40 و 60 و 80 على إنتاج laccases التأسيسية (في غياب VA) و laccases القابلة للحث (في وجود VA) بواسطة Botryosphaeria sp. بشكل منفصل. تم فحص نشاط اللاكيز باستخدام ركيزتين مفترضتين من اللاكيز: ABTS (PPO - I) و DMP (PPO - II). زاد جميع Tweens الذين تم فحصهم من إنتاج كل من laccase PPO - I التأسيسي والقابل للحث، لكنهم لم يؤثروا على إنتاج laccase PPO - II التأسيسي. زاد Tweens 60 و 20 من إنتاج laccase PPO - II القابل للحث، بينما لم يتسبب Tween 80 و 40 في أي تأثيرTranslated Description (English)
Botryosphaeria sp. is a ligninolytic fungus that produces laccase. It was previously established that veratryl alcohol (VA) strongly induced laccase production in this ascomyceteous. It is well known that surfactants such as Tween - 80 can increase the production of some fungal exo-enzymes. It has been reported that Tween-80 can also induce laccase production in basidiomycetes. In this work, the influence of Tweens 20, 40, 60 and 80 on the production of constitutive laccases (in the absence of VA) and inducible laccases (in the presence of VA) by Botryosphaeria sp. was evaluated separately. Laccase activity was assayed using two putative laccase substrates: ABTS (PPO - I) and DMP (PPO - II). All of the Tweens examined increased the production of both constitutive and inducible laccase PPO - I, but did not affect the production of constitutive laccase PPO - II. Tweens 60 and 20 increased the production of inducible laccase PPO - II, while Tween 80 and 40 did not cause any effectTranslated Description (French)
Botryosphaeria sp. est un champignon ligninolytique qui produit de la laccase. Il a été précédemment établi que l'alcool vératrylique (AV) induisait fortement la production de laccase chez cet ascomycète. Il est bien connu que les tensioactifs tels que le Tween - 80 peuvent augmenter la production de certaines exo-enzymes fongiques. Il a été rapporté que le Tween-80 peut également induire la production de laccase chez les basidiomycètes. Dans ce travail, l'influence des Tweens 20, 40, 60 et 80 sur la production de laccases constitutives (en l'absence de VA) et de laccases inductibles (en présence de VA) par Botryosphaeria sp. a été évaluée séparément. L'activité laccase a été testée à l'aide de deux substrats de laccase présumés : ABTS (PPO - I) et DMP (PPO - II). Tous les Tweens examinés ont augmenté la production de la laccase constitutive et inductible PPO - I, mais n'ont pas affecté la production de laccase constitutive PPO - II. Les Tweens 60 et 20 ont augmenté la production de laccase inductible PPO - II, tandis que les Tween 80 et 40 n'ont causé aucun effetTranslated Description (Spanish)
Botryosphaeria sp. es un hongo ligninolítico que produce lacasa. Anteriormente se estableció que el alcohol veratrílico (VA) inducía fuertemente la producción de lacasa en este ascomiceto. Es bien sabido que los tensioactivos como Tween - 80 pueden aumentar la producción de algunas exoenzimas fúngicas. Se ha informado que Tween-80 también puede inducir la producción de lacasa en basidiomicetos. En este trabajo, se evaluó por separado la influencia de Tweens 20, 40, 60 y 80 en la producción de lacasas constitutivas (en ausencia de VA) y lacasas inducibles (en presencia de VA) por Botryosphaeria sp. La actividad de la lacasa se evaluó utilizando dos supuestos sustratos de lacasa: ABTS (PPO - I) y DMP (PPO - II). Todos los preadolescentes examinados aumentaron la producción de lacasa constitutiva e inducible PPO - I, pero no afectaron la producción de lacasa constitutiva PPO - II. Los preadolescentes 60 y 20 aumentaron la producción de lacasa inducible PPO - II, mientras que los preadolescentes 80 y 40 no causaron ningún efectoFiles
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Additional details
Additional titles
- Translated title (Arabic)
- تأثير توين على إنتاج laccases التأسيسية والاستقرائية بواسطة Botryosphaeria sp
- Translated title (English)
- Influence of Tween on the production of constitutive and inductive laccases by Botryosphaeria sp
- Translated title (French)
- Influence de Tween sur la production de laccases constitutives et inductives par Botryosphaeria sp
- Translated title (Spanish)
- Influencia de Tween en la producción de lacasas constitutivas e inductivas por Botryosphaeria sp
Identifiers
- Other
- https://openalex.org/W1994112294
- DOI
- 10.4025/actascibiolsci.v26i4.1536
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