Published July 1, 2014
| Version v1
Publication
Open
Vascular smooth muscle cell culture in microfluidic devices
Creators
- 1. State Key Laboratory of Transducer Technology
- 2. Chinese Academy of Sciences
- 3. Institute of Electronics
- 4. Chinese PLA General Hospital
- 5. Peking University People's Hospital
- 6. Peking University
Description
This paper presents a microfluidic device enabling culture of vascular smooth muscle cells (VSMCs) where extracellular matrix coating, VSMC seeding, culture, and immunostaining are demonstrated in a tubing-free manner. By optimizing droplet volume differences between inlets and outlets of micro channels, VSMCs were evenly seeded into microfluidic devices. Furthermore, the effects of extracellular matrix (e.g., collagen, poly-l-Lysine (PLL), and fibronectin) on VSMC proliferation and phenotype expression were explored. As a platform technology, this microfluidic device may function as a new VSMC culture model enabling VSMC studies.
Translated Descriptions
⚠️
This is an automatic machine translation with an accuracy of 90-95%
Translated Description (Arabic)
تقدم هذه الورقة جهازًا مائعيًا دقيقًا يتيح زراعة خلايا العضلات الملساء الوعائية (VSMCs) حيث يتم عرض طلاء المصفوفة خارج الخلية، وبذر VSMC، والزراعة، والتلوين المناعي بطريقة خالية من الأنابيب. من خلال تحسين اختلافات حجم القطرات بين مداخل ومخارج القنوات الصغيرة، تم زرع VSMCs بالتساوي في أجهزة الموائع الدقيقة. علاوة على ذلك، تم استكشاف تأثيرات المصفوفة خارج الخلية (على سبيل المثال، الكولاجين، بولي لايسين (PLL)، والفيبرونيكتين) على تكاثر VSMC والتعبير عن النمط الظاهري. كتقنية منصة، قد يعمل هذا الجهاز المائعي الدقيق كنموذج ثقافة VSMC جديد يمكّن دراسات VSMC.Translated Description (English)
This paper presents a microfluidic device enabling culture of vascular smooth muscle cells (VSMCs) where extracellular matrix coating, VSMC seeding, culture, and immunostaining are demonstrated in a tubing-free manner. By optimizing droplet volume differences between inlets and outlets of micro channels, VSMCs were evenly seeded into microfluidic devices. Furthermore, the effects of extracellular matrix (e.g., collagen, poly-l-Lysine (PLL), and fibronectin) on VSMC proliferation and phenotype expression were explored. As a platform technology, this microfluidic device may function as a new VSMC culture model enabling VSMC studies.Translated Description (French)
Cet article présente un dispositif microfluidique permettant la culture de cellules musculaires lisses vasculaires (CMLV) où le revêtement de la matrice extracellulaire, l'ensemencement, la culture et l'immunocoloration des CMLV sont démontrés d'une manière sans tubulure. En optimisant les différences de volume des gouttelettes entre les entrées et les sorties des microcanaux, les CMLV ont été ensemencées uniformément dans des dispositifs microfluidiques. De plus, les effets de la matrice extracellulaire (par exemple, le collagène, la poly-l-Lysine (PLL) et la fibronectine) sur la prolifération des CMLV et l'expression du phénotype ont été explorés. En tant que technologie de plate-forme, ce dispositif microfluidique peut fonctionner comme un nouveau modèle de culture VSMC permettant des études VSMC.Translated Description (Spanish)
Este documento presenta un dispositivo microfluídico que permite el cultivo de células musculares lisas vasculares (VSMC) donde el recubrimiento de la matriz extracelular, la siembra de VSMC, el cultivo y la inmunotinción se demuestran sin tubos. Al optimizar las diferencias de volumen de gotas entre las entradas y salidas de los microcanales, las VSMC se sembraron uniformemente en dispositivos microfluídicos. Además, se exploraron los efectos de la matriz extracelular (por ejemplo, colágeno, poli-l-lisina (PLL) y fibronectina) sobre la proliferación de VSMC y la expresión del fenotipo. Como tecnología de plataforma, este dispositivo microfluídico puede funcionar como un nuevo modelo de cultivo de VSMC que permita estudios de VSMC.Files
1.4893914.pdf
Files
(93 Bytes)
| Name | Size | Download all |
|---|---|---|
|
md5:b0d506893d4802090edf1644f5f082cd
|
93 Bytes | Preview Download |
Additional details
Additional titles
- Translated title (Arabic)
- زراعة الخلايا العضلية الملساء الوعائية في أجهزة الموائع الدقيقة
- Translated title (English)
- Vascular smooth muscle cell culture in microfluidic devices
- Translated title (French)
- Culture de cellules musculaires lisses vasculaires dans des dispositifs microfluidiques
- Translated title (Spanish)
- Cultivo de células de músculo liso vascular en dispositivos microfluídicos
Identifiers
- Other
- https://openalex.org/W2011796756
- DOI
- 10.1063/1.4893914
References
- https://openalex.org/W143835407
- https://openalex.org/W1512126150
- https://openalex.org/W1538894879
- https://openalex.org/W1973089587
- https://openalex.org/W1981280006
- https://openalex.org/W1982630129
- https://openalex.org/W1991952399
- https://openalex.org/W1997361365
- https://openalex.org/W2001019345
- https://openalex.org/W2001352820
- https://openalex.org/W2001374505
- https://openalex.org/W2001460285
- https://openalex.org/W2001607419
- https://openalex.org/W2005345591
- https://openalex.org/W2007699685
- https://openalex.org/W2008681436
- https://openalex.org/W2010754054
- https://openalex.org/W2010846389
- https://openalex.org/W2020095634
- https://openalex.org/W2026194129
- https://openalex.org/W2026949179
- https://openalex.org/W2028183845
- https://openalex.org/W2030549786
- https://openalex.org/W2031273706
- https://openalex.org/W2033816998
- https://openalex.org/W2043938126
- https://openalex.org/W2044062630
- https://openalex.org/W2049550437
- https://openalex.org/W2050944525
- https://openalex.org/W2057360288
- https://openalex.org/W2058977087
- https://openalex.org/W2061892392
- https://openalex.org/W2065911550
- https://openalex.org/W2072110232
- https://openalex.org/W2072492784
- https://openalex.org/W2077248355
- https://openalex.org/W2077499929
- https://openalex.org/W2081697226
- https://openalex.org/W2086187432
- https://openalex.org/W2090073018
- https://openalex.org/W2092941484
- https://openalex.org/W2108868865
- https://openalex.org/W2110972796
- https://openalex.org/W2125414597
- https://openalex.org/W2126275439
- https://openalex.org/W2127862291
- https://openalex.org/W2131328415
- https://openalex.org/W2137485439
- https://openalex.org/W2138029925
- https://openalex.org/W2155835199
- https://openalex.org/W2158053452
- https://openalex.org/W2160322193
- https://openalex.org/W2290691325
- https://openalex.org/W2320689805
- https://openalex.org/W94121859